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Image Search Results
Journal: bioRxiv
Article Title: Systematic design of multiplex methylation-specific qPCR for cancer related biomarkers
doi: 10.1101/2024.12.09.627654
Figure Lengend Snippet: A. The 4-plex assay (4+G) shows a higher accumulated methylation signal (indicated by a lower mean Cq value) compared to the singleplex assay (1+G) for the positive control (hypermethylated DNA). B . qPCR readout of the 4-plex (blue) and 1-plex (purple) assays on the positive control (hypermethylated DNA). ROX (red) is used as a reference dye, and GAPDH (green) is used to report DNA input quantity and normalize methylation detection.
Article Snippet: 10 ng of bisulfite-converted DNA input were used in 10 µL qPCR reactions with 2x EvaGreen qPCR MasterMix with
Techniques: Plex Assay, Methylation, Singleplex Assay, Positive Control